Bmi1 antibody
Catalogue Number: GTX114008-GTX
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX114008 Image
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Bmi1 antibody detects Bmi1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded dog lung. Bmi1 stained by Bmi1 antibody (GTX114008) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Bmi1 antibody detects Bmi1 protein at nucleus by immunofluorescent analysis. Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Bmi1 stained by Bmi1 antibody (GTX114008) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar= 10 µm.
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Bmi1 antibody detects Bmi1 protein at nucleus by immunofluorescent analysis. Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Bmi1 stained by Bmi1 antibody (GTX114008) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 µm.
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Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Bmi1 antibody (GTX114008) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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BMI1 antibody [N1C3-2] detects BMI1 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 15 min. Green: BMI1 protein stained by BMI1 antibody (GTX114008) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
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Bmi1 antibody immunoprecipitates Bmi1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of Bmi1 protein expressing 293T cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of Bmi1 protein by 2.5 µg of Bmi1 antibody (GTX114008) 10% SDS-PAGE The immunoprecipitated Bmi1 protein was detected by Bmi1 antibody (GTX114008) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
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Bmi1 antibody detects BMI1 protein by western blot analysis. A. 30 µg THP-1 whole cell lysate/extract B. 30 µg HL-60 whole cell lysate/extract 10% SDS-PAGE Bmi1 antibody (GTX114008) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Bmi1 antibody detects BMI1 protein by western blot analysis. A. 30 µg C8D30 whole cell lysate/extract B. 30 µg BCL-1 whole cell lysate/extract C. 30 µg Raw264.7 whole cell lysate/extract 10% SDS-PAGE Bmi1 antibody (GTX114008) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Bmi1 antibody detects Bmi1 protein by western blot analysis. A. 30 µg K562 whole cell lysate/extract B. 30 µg THP-1 whole cell lysate/extract 10% SDS-PAGE Bmi1 antibody (GTX114008) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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The data was published in the journal J Biomed Sci in 2016.PMID: 27863490
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The data was published in the journal J Biomed Sci in 2016.PMID: 27863490
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The data was published in the journal BMC Cancer in 2017.PMID: 28629331
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The data was published in the journal Cell Death Dis in 2015. PMID: 26136074
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Bmi1 antibody detects Bmi1 protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. Bmi1 antibody (GTX114008) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Bmi1 antibody detects Bmi1 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Bmi1 antibody (GTX114008) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 37 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | BMI1 proto-oncogene, polycomb ring finger , FLVI2/BMI1 , PCGF4 , RNF51 , flvi-2/bmi-1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Bmi1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Additional Text
Uniprot ID
P35226
Gene ID
100532731; 648
Purification
Affinity Purified
Molecular Weight
37
Gene Name
BMI1, COMMD3-BMI1
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX114008 Datasheet
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