Aconitase 2 antibody [N1N3]
Catalogue Number: GTX114233-GTX
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Aconitase 2 antibody detects Aconitase 2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody (GTX114233) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunoprecipitation of Aconitase 2 protein from HeLa whole cell extracts using 5 µg of Aconitase 2 antibody [N1N3] (GTX114233). Western blot analysis was performed using Aconitase 2 antibody [N1N3] (GTX114233). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Aconitase 2 antibody [N1N3] (GTX114233) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Aconitase 2 antibody [N1N3] (GTX114233) at 1:300 dilution.
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Sample (20 µg of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX114233 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Sample (30 µg of whole cell lysate) A: zebrafish eye 7.5% SDS PAGE GTX114233 diluted at 1:1000
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Aconitase 2 antibody [N1N3] detects Aconitase 2 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: Aconitase 2 protein stained by Aconitase 2 antibody [N1N3] (GTX114233) diluted at 1:1000. Red: MitoTrackerR Red CMXRos, a mitochondria tracker. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
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Sample (30 µg of whole cell lysate) A: JurKat 7.5% SDS PAGE Aconitase 2 antibody GTX114233 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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The data was published in the journal Biomolecules in 2019. PMID: 31614639
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Aconitase 2 antibody [N1N3] detects Aconitase 2 protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Aconitase 2 antibody [N1N3] (GTX114233) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Aconitase 2 antibody [N1N3] detects Aconitase 2 protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Aconitase 2 antibody [N1N3] (GTX114233) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Aconitase 2 antibody [N1N3] detects Aconitase 2 protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Aconitase 2 antibody [N1N3] (GTX114233) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Aconitase 2 antibody [N1N3] detects Aconitase 2 protein at mitochondria on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. Aconitase 2 antibody [N1N3] (GTX114233) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 85 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | aconitase 2 , ACONM , HEL-S-284 , ICRD , OCA8 , OPA9 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human Aconitase 2. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: The protein encoded by this gene belongs to the aconitase/IPM isomerase family. It is an enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle. This protein is encoded in the nucleus and functions in the mitochondrion. It was found to be one of the mitochondrial matrix proteins that are preferentially degraded by the serine protease 15(PRSS15), also known as Lon protease, after oxidative modification. [provided by RefSeq]
Additional Text
Gene Name
ACO2
Uniprot ID
Q99798
Gene ID
50
Purification
Affinity Purified
Molecular Weight
85
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX114233 Datasheet
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