ETFA antibody
Catalogue Number: GTX114392-GTX
GTX114392 Image
GTX114392 Image
GTX114392 Image
GTX114392 Image
GTX114392 Image
GTX114392 Image
GTX114392 Image
GTX114392 Image
GTX114392 Image
GTX114392 Image
GTX114392 Image
GTX114392 Image
GTX114392 Image
GTX114392 Image
Immunoprecipitation of ETFA protein from HepG2 whole cell extracts using 5 µg of ETFA antibody (GTX114392). Western blot analysis was performed using ETFA antibody (GTX114392). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX114392 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX114392) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunofluorescence analysis of methanol-fixed HeLa, using ETFA(GTX114392) antibody at 1:200 dilution.
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Immunohistochemical analysis of agarose-embedded zebrafish embryo, using ETFA antibody (GTX114392) at 1:100 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)
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ETFA antibody detects ETFA protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract 10 % SDS-PAGE ETFA antibody (GTX114392) dilution: 1:1000
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ETFA antibody detects ETFA protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 10 % SDS-PAGE ETFA antibody (GTX114392) dilution: 1:1000
GTX114392 Image
ETFA antibody detects ETFA protein by Western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX114392) diluted by 1:1000.
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Various tissue extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX114392) diluted at 1:500.
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Wild-type (WT) and ETFA knockout (KO) 293T cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA antibody (GTX114392) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX114392 Image
ETFA antibody detects ETFA protein at mitochondria on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ETFA antibody (GTX114392) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX114392 Image
ETFA antibody detects ETFA protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ETFA antibody (GTX114392) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX114392 Image
ETFA antibody detects ETFA protein at mitochondria on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. ETFA antibody (GTX114392) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX114392 Image
ETFA antibody detects ETFA protein at mitochondria on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. ETFA antibody (GTX114392) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 35 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | electron transfer flavoprotein subunit alpha , EMA , GA2 , MADD |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human ETFA. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB, IHC |
Description
Description: ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Additional Text
Gene Name
ETFA
Uniprot ID
P13804
Purification
Affinity Purified
Gene ID
2108
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
35
Concentration
1 mg/ml
Application Notes
WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX114392 Datasheet
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