GNB1 antibody
Catalogue Number: GTX114442-GTX
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GNB1 antibody detects GNB1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GNB1 protein stained by GNB1 antibody (GTX114442) diluted at 1:500. Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000. Blue: Hoechst 33342 staining.
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Immunoprecipitation of GNB1 protein from 293T whole cell extracts using 5 µg of GNB1 antibody (GTX114442). Western blot analysis was performed using GNB1 antibody (GTX114442). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using GNB1 antibody (GTX114442) at 1:300 dilution.
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Immunohistochemical analysis (whole mount) of zebrafish embryo, using GNB1 antibody (GTX114442) at 1:200 dilution.
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Sample (50 µg of whole cell lysate) A: mouse brain 10% SDS PAGE GTX114442 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GNB1 antibody detects GNB1 protein at cell membrane in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. GNB1 antibody (GTX114442) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GNB1 antibody detects GNB1 protein at cell membrane in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. GNB1 antibody (GTX114442) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Various tissue extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNB1 antibody (GTX114442) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various tissue extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNB1 antibody (GTX114442) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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The data was published in the journal Front Physiol in 2019. PMID: 31992990
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The data was published in the journal eNeuro in 2018. PMID: 29911170
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Immunohistochemical analysis of paraffin-embedded human lung papillory adenocarcinoma, using GNB1(GTX114442) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNB1 antibody (GTX114442) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 37 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | G protein subunit beta 1 , MRD42 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human GNB1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB, IHC-Fr, IHC-WM, PLA |
Description
Description: Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals. [provided by RefSeq]
Additional Text
Gene Name
GNB1
Uniprot ID
P62873
Purification
Affinity Purified
Gene ID
2782
Molecular Weight
37
Concentration
1.32 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX114442 Datasheet
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