TFIIF antibody
Catalogue Number: GTX114455-GTX
GTX114455 Image
GTX114455 Image
GTX114455 Image
GTX114455 Image
GTX114455 Image
Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or anti-TFIIF antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter or MyoD.
GTX114455 Image
TFIIF antibody immunoprecipitates TFIIF protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of TFIIF protein expressing 293T cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of TFIIF by 2.5 µg of TFIIF antibody (GTX114455) 7.5% SDS-PAGE The immunoprecipitated TFIIF protein was detected by TFIIF antibody (GTX114455) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
GTX114455 Image
Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE GTX114455 diluted at 1:3000
GTX114455 Image
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa 7.5% SDS PAGE GTX114455 diluted at 1:3000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 58 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | general transcription factor IIF subunit 1 , BTF4 , RAP74 , TF2F1 , TFIIF |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human TFIIF. The exact sequence is proprietary. |
| Application: | IP, ChIP, WB |
Description
Description: TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.
Additional Text
Gene Name
GTF2F1
Uniprot ID
P35269
Purification
Affinity Purified
Gene ID
2962
Molecular Weight
58
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX114455 Datasheet
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