Brn2 antibody
Catalogue Number: GTX114650-GTX
GTX114650 Image
GTX114650 Image
GTX114650 Image
GTX114650 Image
GTX114650 Image
GTX114650 Image
GTX114650 Image
GTX114650 Image
GTX114650 Image
Brn2 antibody detects Brn2 protein expression at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX114650 Image
Brn2 antibody immunoprecipitates Brn2 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 µg preimmune rabbit IgG B. 5 µg of Brn2 antibody (GTX114650) The precipitated DNA was detected by PCR with primer set targeting to MITF promoter.
GTX114650 Image
Brn2 antibody immunoprecipitates Brn2 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 50 µg 293T whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of Brn2 protein by 2 µg of Brn2 antibody (GTX114650) 10% SDS-PAGE The immunoprecipitated Brn2 protein was detected by Brn2 antibody (GTX114650) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX114650 Image
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Brn2 antibody (GTX114650) diluted at 1:1000.
GTX114650 Image
Brn2 antibody detects Brn2 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Brn2 antibody (GTX114650) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX114650 Image
Brn2 antibody detects Brn2 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Brn2 antibody (GTX114650) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX114650 Image
Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using Brn2(GTX114650) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX114650 Image
Brn2 antibody detects Brn2 protein at nucleus by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Brn2 protein stained by Brn2 antibody (GTX114650) diluted at 1:1000. Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Scale bar = 10 µm.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 47 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | POU class 3 homeobox 2 , BRN2 , N-Oct3 , OCT7 , OTF-7 , OTF7 , POUF3 , brn-2 , oct-7 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the C-terminus region of human Brn2. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB, IHC-Fr |
Description
Description: POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).[supplied by OMIM]
Additional Text
Gene Name
POU3F2
Concentration
0.2 mg/ml
Purification
Affinity Purified
Gene ID
5454
Molecular Weight
47
Uniprot ID
P20265
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX114650 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations