RPL9 antibody
Catalogue Number: GTX114728-GTX
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GTX114728 Image
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RPL9 antibody detects RPL9 protein at retinal pigment epithelium in mouse eye by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. RPL9 antibody (GTX114728) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Immunofluorescence analysis of methanol-fixed HeLa, using RPL9(GTX114728) antibody at 1:200 dilution.
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Immunohistochemical analysis (whole mount) of zebrafish embryo, using RPL9 antibody (GTX114728) at 1:200 dilution.
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Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE GTX114728 diluted at 1:1000
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Sample (30 µg of whole cell lysate) A: Zebrafish muscle 12% SDS PAGE GTX114728 diluted at 1:1000
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Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 (GTX27900) C: HCT116 12% SDS PAGE GTX114728 diluted at 1:1000
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The data was published in the journal Cell Death Dis in 2016. PMID: 27253413
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Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using RPL9 antibody(GTX114728) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RPL9 antibody(GTX114728) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 22 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | ribosomal protein L9 , L9 , NPC-A-16 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human RPL9. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC-WM |
Description
Description: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L6P family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
Additional Text
Gene Name
RPL9
Uniprot ID
P32969
Gene ID
6133
Concentration
0.8 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
22
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX114728 Datasheet
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