MRPL12 antibody [N1C3]
Catalogue Number: GTX114731-GTX
GTX114731 Image
GTX114731 Image
GTX114731 Image
GTX114731 Image
GTX114731 Image
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with MRPL12 antibody [N1C3] (GTX114731) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE GTX114731 diluted at 1:1000
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MRPL12 antibody [N1C3] detects MRPL12 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min. Green: MRPL12 protein stained by MRPL12 antibody [N1C3] (GTX114731) diluted at 1:500. Blue: Hoechst 33342 staining.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using MRPL12(GTX114731) antibody at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 21 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | mitochondrial ribosomal protein L12 , 5c5-2 , L12mt , MRP-L31/34 , MRPL7 , MRPL7/L12 , RPML12 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human MRPL12. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, PLA |
Description
Description: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq]
Additional Text
Gene Name
MRPL12
Gene ID
6182
Uniprot ID
P52815
Concentration
0.56 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
21
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX114731 Datasheet
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