SATB1 antibody [C2C3], C-term
Catalogue Number: GTX114738-GTX
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Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Cross-linked ChIP was performed with Jurkat chromatin extract and 5 µg of either control rabbit IgG or anti-SATB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to BCL2 promoter.
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SATB1 antibody immunoprecipitates SATB1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 µg of preimmune rabbit IgG B. Immunoprecipitation of SATB1 protein by 2 µg of SATB1 antibody (GTX114738) 7.5% SDS-PAGE The immunoprecipitated SATB1 protein was detected by SATB1 antibody (GTX114738) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:40000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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SATB1 antibody [C2C3], C-term detects SATB1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. SATB1 stained by SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:500. Antigen Retrieval: Citra
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SATB1 antibody [C2C3], C-term detects SATB1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SATB1 stained by SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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Immunoprecipitation of SATB1 protein from 293T whole cell extract using 5 µg of SATB1 antibody [C2C3], C-term (GTX114738). Western blot analysis was performed using SATB1 antibody [C2C3], C-term (GTX114738). EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).
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SATB1 antibody [C2C3], C-term detects SATB1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. SATB1 stained by SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:500. Antigen Retrieval: Citrate
GTX114738 Image
Immunoprecipitation of SATB1 protein from 293T whole cell extract using 5 µg of SATB1 antibody [C2C3], C-term (GTX114738). Western blot analysis was performed using SATB1 antibody [C2C3], C-term (GTX114738). EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SATB1(GTX114738) antibody at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 86 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | SATB homeobox 1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the C-terminus region of human SATB1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB |
Description
Description: This gene encodes a matrix protein which binds nuclear matrix and scaffold-associating DNAs through a unique nuclear architecture. The protein recruits chromatin-remodeling factors in order to regulate chromatin structure and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Additional Text
Gene Name
SATB1
Uniprot ID
Q01826
Concentration
0.36 mg/ml
Purification
Affinity Purified
Gene ID
6304
Molecular Weight
86
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:10000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX114738 Datasheet
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