HLTF antibody [N2C1], Internal
Catalogue Number: GTX114776-GTX
GTX114776 Image
GTX114776 Image
GTX114776 Image
GTX114776 Image
GTX114776 Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HLTF(GTX114776) antibody at 1:500 dilution.
GTX114776 Image
Immunohistochemical analysis of paraffin-embedded MB157 xenograft, using HLTF(GTX114776) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX114776 Image
HLTF antibody immunoprecipitates HLTF protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of HLTF protein by 2 µg of HLTF antibody (GTX114776) 7.5% SDS-PAGE The immunoprecipitated HLTF protein was detected by HLTF antibody (GTX114776) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX114776 Image
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE GTX114776 diluted at 1:5000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 114 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | helicase like transcription factor , HIP116 , HIP116A , HLTF1 , RNF80 , SMARCA3 , SNF2L3 , ZBU1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human HLTF. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq]
Additional Text
Gene Name
HLTF
Uniprot ID
Q14527
Purification
Affinity Purified
Gene ID
6596
Molecular Weight
114
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Concentration
0.62 mg/ml
GTX114776 Datasheet
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