SMARCC1 antibody [C2C3], C-term
Catalogue Number: GTX114777-GTX
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Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term (GTX114777) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Cross-linked ChIP was performed with MF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 µg of either control rabbit IgG or anti-SMARCC1 antibody. The precipitated DNA was detected by PCR with primer set targeting to ESR1 or Sat2.
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Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SMARCC1(GTX114777) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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SMARCC1 antibody immunoprecipitates SMARCC1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of SMARCC1 protein expressing 293T cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of SMARCC1 by 2.5 µg of SMARCC1 antibody (GTX114777) 5% SDS-PAGE The immunoprecipitated SMARCC1 protein was detected by SMARCC1 antibody (GTX114777) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
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SMARCC1 antibody [C2C3], C-term detects SMARCC1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMARCC1 stained by SMARCC1 antibody [C2C3], C-term (GTX114777) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.
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Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term (GTX114777) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Wild-type (WT) and SMARCC1 knockout (KO) HeLa cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMARCC1 antibody [C2C3], C-term (GTX114777) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using SMARCC1(GTX114777) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using SMARCC1(GTX114777) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using SMARCC1(GTX114777) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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SMARCC1 antibody [C2C3], C-term detects SMARCC1 protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. SMARCC1 antibody [C2C3], C-term (GTX114777) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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SMARCC1 antibody [C2C3], C-term detects SMARCC1 protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. SMARCC1 antibody [C2C3], C-term (GTX114777) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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SMARCC1 antibody [C2C3], C-term detects SMARCC1 protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. SMARCC1 antibody [C2C3], C-term (GTX114777) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 123 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 1 , BAF155 , CRACC1 , Rsc8 , SRG3 , SWI3 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the C-terminus region of human SMARCC1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB |
Description
Description: The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. [provided by RefSeq]
Additional Text
Gene Name
SMARCC1
Uniprot ID
Q92922
Gene ID
6599
Concentration
0.77 mg/ml
Purification
Affinity Purified
Molecular Weight
123
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX114777 Datasheet
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