Munc18-1 antibody
Catalogue Number: GTX114809-GTX
GTX114809 Image
GTX114809 Image
GTX114809 Image
GTX114809 Image
GTX114809 Image
Munc18-1 antibody detects Munc18-1 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: Munc18-1 protein stained by Munc18-1 antibody (GTX114809) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX114809 Image
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Munc18-1 antibody (GTX114809) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX114809 Image
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using STXBP1(GTX114809) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
GTX114809 Image
Sample (30 ug of whole cell lysate) A: 293T B: A431 (GTX27909) 7.5% SDS PAGE GTX114809 diluted at 1:3000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 68 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | syntaxin binding protein 1 , MUNC18-1 , NSEC1 , P67 , RBSEC1 , UNC18 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the C-terminus region of human Munc18-1. The exact sequence is proprietary. |
| Application: | ICC, IF, IP, WB |
Description
Description: This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described. [provided by RefSeq]
Additional Text
Gene ID
6812
Gene Name
STXBP1
Uniprot ID
P61764
Concentration
0.96 mg/ml
Purification
Affinity Purified
Molecular Weight
68
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX114809 Datasheet
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