UQCRC2 antibody
Catalogue Number: GTX114873-GTX
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GTX114873 Image
GTX114873 Image
GTX114873 Image
GTX114873 Image
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Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC2 antibody (GTX114873) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with UQCRC2 antibody (GTX114873) diluted at 1:10000 and competitor's antibody (14742-1-AP) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
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Immunofluorescence analysis of paraformaldehyde-fixed A431, using UQCRC2(GTX114873) antibody at 1:500 dilution.
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UQCRC2 antibody immunoprecipitates UQCRC2 protein in IP experiments. IP samples: HepG2 whole cell extract A. 35 µg HepG2 whole cell extract B. Control with 4 µg of preimmune Rabbit IgG C. Immunoprecipitation of UQCRC2 protein by 4 µg UQCRC2 antibody (GTX114873) 10 % SDS-PAGE The immunoprecipitated UQCRC2 protein was detected by UQCRC2 antibody (GTX114873) diluted at 1:5000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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UQCRC2 antibody detects UQCRC2 protein at cytoplasm on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. UQCRC2 antibody (GTX114873) dilution: 1:250. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.01% Thimerosal |
| Molecular Weight: | 48 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | ubiquinol-cytochrome c reductase core protein 2 , MC3DN5 , QCR2 , UQCR2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human UQCRC2. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB, PLA |
Description
Description: This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. The core protein 2 is required for the assembly of the complex.
Additional Text
Gene Name
UQCRC2
Uniprot ID
P22695
Purification
Affinity Purified
Gene ID
7385
Molecular Weight
48
Concentration
1.37 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX114873 Datasheet
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