USP14 antibody
Catalogue Number: GTX115187-GTX
GTX115187 Image
GTX115187 Image
GTX115187 Image
GTX115187 Image
GTX115187 Image
GTX115187 Image
GTX115187 Image
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with USP14 antibody (GTX115187) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX115187 Image
USP14 antibody detects USP14 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: USP14 protein stained by USP14 antibody (GTX115187) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ Tuj1 antibody [GT886] (GTX631830) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX115187 Image
USP14 antibody detects USP14 protein by immunofluorescent analysis. Sample: DIV14 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: USP14 protein stained by USP14 antibody (GTX115187) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX115187 Image
Wild-type (WT) and USP14 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with USP14 antibody (GTX115187) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX115187 Image
Sample (30 ug of whole cell lysate) A: A431 (GTX27909) 7.5% SDS PAGE GTX115187 diluted at 1:1000
GTX115187 Image
Immunohistochemical analysis of paraffin-embedded human normal liver, using USP14(GTX115187) antibody at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 56 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | ubiquitin specific peptidase 14 , TGT |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human USP14. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
Additional Text
Gene Name
USP14
Gene ID
9097
Uniprot ID
P54578
Purification
Affinity Purified
Molecular Weight
56
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX115187 Datasheet
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