SETDB1 antibody [N1], N-term
Catalogue Number: GTX115305-GTX
GTX115305 Image
GTX115305 Image
GTX115305 Image
GTX115305 Image
GTX115305 Image
Whole cell extract (30 µg) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX115305 Image
Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or anti-SETDB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to P53P2.
GTX115305 Image
SETDB1 antibody [N1], N-term immunoprecipitates SETDB1 protein in IP experiments. IP samples: HeLa nuclear extract A. Control with 4 µg of preimmune Rabbit IgG B. Immunoprecipitation of SETDB1 protein by 4 µg SETDB1 antibody [N1], N-term (GTX115305) 5 % SDS-PAGE The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
GTX115305 Image
HeLa whole cell and nuclear extract (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (GTX115305) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.01% Thimerosal |
| Molecular Weight: | 143 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | SET domain bifurcated histone lysine methyltransferase 1 , ESET , H3-K9-HMTase4 , KG1T , KMT1E , TDRD21 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human SETDB1. The exact sequence is proprietary. |
| Application: | IHC-P, IP, ChIP, WB |
Description
Description: This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.
Additional Text
Gene Name
SETDB1
Uniprot ID
Q15047
Concentration
0.53 mg/ml
Purification
Affinity Purified
Gene ID
9869
Molecular Weight
143
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX115305 Datasheet
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