SCGN antibody [N2C3]
Catalogue Number: GTX115443-GTX
GTX115443 Image
GTX115443 Image
GTX115443 Image
GTX115443 Image
GTX115443 Image
GTX115443 Image
GTX115443 Image
GTX115443 Image
SCGN antibody [N2C3] detects SCGN protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: SCGN protein stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX115443 Image
Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SCGN antibody [N2C3] (GTX115443) diluted at 1:500.
GTX115443 Image
SCGN antibody [N2C3] detects SCGN protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green:SCGN protein stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
GTX115443 Image
SCGN antibody [N2C3] detects SCGN Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: SCGN stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250. Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
GTX115443 Image
SCGN antibody [N2C3] detects SCGN protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SCGN stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX115443 Image
Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE GTX115443 diluted at 1:1000
GTX115443 Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SCGN(GTX115443) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 32 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | secretagogin, EF-hand calcium binding protein , CALBL , DJ501N12.8 , SECRET , SEGN , setagin |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human SCGN. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq]
Additional Text
Gene Name
SCGN
Molecular Weight
32
Gene ID
10590
Uniprot ID
O76038
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX115443 Datasheet
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