CREST antibody [N1C2]
Catalogue Number: GTX115785-GTX
GTX115785 Image
GTX115785 Image
GTX115785 Image
GTX115785 Image
GTX115785 Image
Immunoprecipitation of CREST protein from MCF-7 whole cell extracts using 5 µg of CREST antibody [N1C2] (GTX115785). Western blot analysis was performed using CREST antibody [N1C2] (GTX115785). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Immunofluorescence analysis of paraformaldehyde-fixed A431, using CREST(GTX115785) antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using CREST(GTX115785) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX115785 Image
Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with CREST antibody [N1C2] (GTX115785) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 43 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | SS18L1 subunit of BAF chromatin remodeling complex , CREST , LP2261 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human CREST. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: Synovial sarcomas occur most frequently in the extremities around large joints. More than 90% of cases have a recurrent and specific chromosomal translocation, t(X;18)(p11.2;q11.2), in which the 5-prime end of the SS18 gene (MIM 600192) is fused in-frame to the 3-prime end of the SSX1 (MIM 312820), SSX2 (MIM 300192), or SSX4 (MIM 300326) gene. The SS18L1 gene is homologous to SS18.[supplied by OMIM]
Additional Text
Gene Name
SS18L1
Uniprot ID
O75177
Concentration
1.4 mg/mL
Purification
Affinity Purified
Gene ID
26039
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
43
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX115785 Datasheet
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