APC antibody [C3], C-term
Catalogue Number: GTX116009-GTX
GTX116009 Image
GTX116009 Image
GTX116009 Image
GTX116009 Image
GTX116009 Image
GTX116009 Image
GTX116009 Image
APC antibody [C3], C-term detects APC protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: APC protein stained by APC antibody [C3], C-term (GTX116009) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.
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Immunofluorescence analysis of methanol-fixed A431, using APC(GTX116009) antibody at 1:500 dilution.
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APC antibody [C3], C-term detects APC protein at cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. APC antibody [C3], C-term (GTX116009) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX116009 Image
APC antibody [C3], C-term detects APC protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated). Sample: Paraffin-embedded human colon cancer. APC stained by APC antibody [C3], C-term (GTX116009) diluted at 1:500. Antigen Retrieval: Citrate buffer, 20 min
GTX116009 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with APC antibody [C3], C-term (GTX116009) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX116009 Image
Non-transfected (-) and transfected (+) unboiled HeLa whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with APC antibody [C3], C-term (GTX116009) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 312 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | APC regulator of WNT signaling pathway , BTPS2 , DP2 , DP2.5 , DP3 , GS , PPP1R46 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human APC. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC |
Description
Description: This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq]
Additional Text
Gene ID
324
Gene Name
APC
Uniprot ID
P25054
Concentration
1.44 mg/mL
Purification
Affinity Purified
Molecular Weight
312
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX116009 Datasheet
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