Geminin antibody
Catalogue Number: GTX116125-GTX
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GTX116125 Image
GTX116125 Image
GTX116125 Image
GTX116125 Image
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Geminin(GTX116125) antibody at 1:500 dilution.
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Immunoprecipitation of Geminin protein from HCT-116 whole cell extracts using 5 µg of Geminin antibody (GTX116125). Western blot analysis was performed using Geminin antibody (GTX116125) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Sample (30 ug of whole cell lysate) A: HCT116 12% SDS PAGE GTX116125 diluted at 1:1000
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The data was published in the journal J Virol in 2019.PMID: 31142673
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Geminin antibody detects Geminin protein at nucleus on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. Geminin antibody (GTX116125) dilution: 1:250. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 24 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | geminin DNA replication inhibitor , Gem , MGORS6 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Full length human Geminin Recombinant protein. |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: Inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.
Additional Text
Gene Name
GMNN
Molecular Weight
24
Uniprot ID
O75496
Gene ID
51053
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX116125 Datasheet
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