TRIM25 antibody
Catalogue Number: GTX116209-GTX
GTX116209 Image
GTX116209 Image
GTX116209 Image
GTX116209 Image
GTX116209 Image
GTX116209 Image
TRIM25 antibody detects TRIM25 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: TRIM25 protein stained by TRIM25 antibody (GTX116209) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
GTX116209 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM25 antibody (GTX116209) diluted at 1:500.
GTX116209 Image
TRIM25 antibody immunoprecipitates TRIM25 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of TRIM25 protein by 2 µg of TRIM25 antibody (GTX116209) 7.5% SDS-PAGE The immunoprecipitated TRIM25 protein was detected by TRIM25 antibody (GTX116209) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX116209 Image
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE GTX116209 diluted at 1:1000
GTX116209 Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using TRIM25(GTX116209) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 71 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | tripartite motif containing 25 , EFP , RNF147 , Z147 , ZNF147 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human TRIM25. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq]
Additional Text
Gene ID
7706
Uniprot ID
Q14258
Gene Name
TRIM25
Purification
Affinity Purified
Molecular Weight
71
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX116209 Datasheet
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