Galectin 1 antibody
Catalogue Number: GTX116411-GTX
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Galectin 1 antibody detects Galectin 1 protein at cytoplasm by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Galectin 1 protein stained by Galectin 1 antibody (GTX116411) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
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Galectin 1 antibody detects Galectin 1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Galectin 1 protein stained by Galectin 1 antibody (GTX116411) diluted at 1:200. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.
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Wild-type (WT) and Galectin 1 knockout (KO) 293T cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX116411) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Immunofluorescence analysis of methanol-fixed A431, using Galectin 1(GTX116411) antibody at 1:200 dilution.
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Galectin 1 antibody immunoprecipitates Galectin 1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 µg whole cell lysate/extract of Galectin 1 antibody expressing HeLa cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of Galectin 1 by 2.5 µg of Galectin 1 antibody (GTX116411) 15% SDS-PAGE The immunoprecipitated Galectin 1 protein was detected by Galectin 1 antibody (GTX116411) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
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Sample (30 µg of whole cell lysate) A: BCL-1 15% SDS PAGE GTX116411 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Galectin 1 antibody detects Galectin 1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 15% SDS-PAGE Galectin 1 antibody (GTX116411) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Sample (30 µg of whole cell lysate) A: NCI-H929 15% SDS PAGE GTX116411 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Galectin 1 antibody (GTX116411) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 15 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | galectin 1 , GAL1 , GBP |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Full length human Galectin 1 Recombinant protein. |
| Application: | ICC, IF, IP, WB |
Description
Description: The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq]
Additional Text
Gene Name
LGALS1
Gene ID
3956
Purification
Affinity Purified
Uniprot ID
P09382
Concentration
1.48 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
15
Application Notes
WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX116411 Datasheet
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