PRMT7 antibody
Catalogue Number: GTX116570-GTX
GTX116570 Image
GTX116570 Image
GTX116570 Image
GTX116570 Image
GTX116570 Image
Cross-linked ChIP was performed with NIH3T3 chromatin extract and 5 µg of either control rabbit IgG or anti-PRMT7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Apex2.
GTX116570 Image
PRMT7 antibody detects PRMT7 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRMT7 protein stained by PRMT7 antibody (GTX116570) diluted at 1:500. Blue: Hoechst 33342 staining.
GTX116570 Image
PRMT7 antibody immunoprecipitates PRMT7 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of PRMT7 protein by 2 µg of PRMT7 antibody (GTX116570) 7.5% SDS-PAGE The immunoprecipitated PRMT7 protein was detected by PRMT7 antibody (GTX116570) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX116570 Image
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using PRMT7(GTX116570) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 78 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | protein arginine methyltransferase 7 , SBIDDS |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human PRMT7. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, ChIP |
Description
Description: Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM]
Additional Text
Gene Name
PRMT7
Purification
Affinity Purified
Gene ID
54496
Uniprot ID
Q9NVM4
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX116570 Datasheet
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