DDX4 antibody [C1C3]
Catalogue Number: GTX116575-GTX
GTX116575 Image
GTX116575 Image
GTX116575 Image
GTX116575 Image
GTX116575 Image
DDX4 antibody [C1C3] detects DDX4 protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. DDX4 antibody [C1C3] (GTX116575) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX116575 Image
Sample (30 µg of whole cell lysate) A: U87-MG 7.5% SDS PAGE GTX116575 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX116575 Image
Mouse tissue extract (30 | 50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX4 antibody [C1C3] (GTX116575) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX116575 Image
Human tissue extracts (30 | 50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DDX4 antibody [C1C3] (GTX116575) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.01% Thimerosal |
| Molecular Weight: | 79 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | DEAD-box helicase 4 , VASA |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the C-terminus region of human DDX4. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC-WM |
Description
Description: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. [provided by RefSeq]
Additional Text
Gene Name
DDX4
Concentration
1.36 mg/ml
Purification
Affinity Purified
Gene ID
54514
Molecular Weight
79
Uniprot ID
Q9NQI0
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX116575 Datasheet
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