SETDB2 antibody [N1N2], N-term
Catalogue Number: GTX116932-GTX
GTX116932 Image
GTX116932 Image
GTX116932 Image
GTX116932 Image
SETDB2 antibody [N1N2], N-term detects SETDB2 protein at nucleus by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SETDB2 protein stained by SETDB2 antibody [N1N2], N-term (GTX116932) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
GTX116932 Image
SETDB2 antibody immunoprecipitates SETDB2 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of SETDB2 protein by 2 µg of SETDB2 antibody (GTX116932) 7.5% SDS-PAGE The immunoprecipitated SETDB2 protein was detected by SETDB2 antibody (GTX116932) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX116932 Image
Sample (30 ug of whole cell lysate) A: SK-N-SH 7.5% SDS PAGE GTX116932 diluted at 1:1000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 82 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | SET domain bifurcated histone lysine methyltransferase 2 , C13orf4 , CLLD8 , CLLL8 , KMT1F |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human SETDB2. The exact sequence is proprietary. |
| Application: | ICC, IF, IP, WB |
Description
Description: Proteins that contain a SET domain, such as SETDB2, modulate gene expression epigenetically through histone H3 (see MIM 601128) methylation. SETDB2 is likely a histone H3 methyltransferase, as it contains both the active site and flanking cysteine residues required for catalytic activity (Zhang et al., 2003 [PubMed 12754510]).[supplied by OMIM]
Additional Text
Gene Name
SETDB2
Uniprot ID
Q96T68
Purification
Affinity Purified
Gene ID
83852
Molecular Weight
82
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX116932 Datasheet
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