LIN7A antibody [N1C3]
Catalogue Number: GTX117114-GTX
GTX117114 Image
GTX117114 Image
GTX117114 Image
GTX117114 Image
GTX117114 Image
GTX117114 Image
GTX117114 Image
LIN7A antibody [N1C3] detects Lin7a protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. LIN7A antibody [N1C3] (GTX117114) dilution: 1:200.
GTX117114 Image
LIN7A antibody [N1C3] detects LIN7A protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green: LIN7A protein stained by LIN7A antibody [N1C3] (GTX117114) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using LIN7A(GTX117114) antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using LIN7A antibody [N1C3] (GTX117114) at 1:300 dilution.
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Sample (30 µg of whole cell lysate) A: MCF-7 12% SDS PAGE GTX117114 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Sample (30 µg of whole cell lysate) A: zebrafish eye 12% SDS PAGE GTX117114 diluted at 1:1000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 26 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | lin-7 homolog A, crumbs cell polarity complex component , LIN-7A , LIN7 , MALS-1 , MALS1 , TIP-33 , VELI1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human LIN7A. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC-WM |
Description
Description: Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells.
Additional Text
Gene Name
LIN7A
Uniprot ID
O14910
Purification
Affinity Purified
Gene ID
8825
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
26
Concentration
1 mg/ml
Application Notes
WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX117114 Datasheet
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