HP1 gamma antibody
Catalogue Number: GTX117561-GTX
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GTX117561 Image
GTX117561 Image
GTX117561 Image
GTX117561 Image
GTX117561 Image
GTX117561 Image
GTX117561 Image
HP1 gamma antibody detects HP1 gamma protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. HP1 gamma antibody (GTX117561) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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HP1 gamma antibody detects HP1 gamma protein at nucleus in mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. HP1 gamma antibody (GTX117561) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Wild-type (WT) and HP1 gamma knockout (KO) HeLa cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with HP1 gamma antibody (GTX117561) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using HP1 gamma(GTX117561) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using HP1 gamma(GTX117561) antibody at 1:250 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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HP1 gamma antibody immunoprecipitates HP1 gamma protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of HP1 gamma protein by 2 µg of HP1 gamma antibody (GTX117561) 15% SDS-PAGE The immunoprecipitated HP1 gamma protein was detected by HP1 gamma antibody (GTX117561) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE GTX117561 diluted at 1:10000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 21 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | chromobox 3 , HECH , HP1-GAMMA , HP1Hs-gamma |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human HP1 gamma. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, WB |
Description
Description: At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene. [provided by RefSeq]
Additional Text
Gene ID
11335
Gene Name
CBX3
Uniprot ID
Q13185
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
21
Concentration
1 mg/ml
Application Notes
WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX117561 Datasheet
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