DCTN1 antibody, C-term
Catalogue Number: GTX11806-GTX
GTX11806 Image
GTX11806 Image
GTX11806 Image
GTX11806 Image
GTX11806 Image
GTX11806 Image
WB analysis of human cerebellum lysate using GTX11806 DCTN1 antibody, C-term. Dilution : 1µg/ml Loading : 35µg protein in RIPA buffer
GTX11806 Image
IHC-P analysis of human cerebellum using GTX11806 DCTN1 antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 2.5µg/ml
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FACS analysis of PFA fixed HeLa cells using GTX11806 DCTN1 antibody, C-term. Blue : Primary antibody Black : Isotype control Permeabilization : 0.5% Triton Dilution : 10µg/ml
GTX11806 Image
ICC/IF analysis of PFA fixed HeLa cells using GTX11806 DCTN1 antibody, C-term. Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 10µg/ml
GTX11806 Image
ICC/IF analysis of PFA fixed U2OS cells using GTX11806 DCTN1 antibody, C-term. Green : Primary antibody Blue : DAPI Negative control : Unimmunized goat IgG Permeabilization : 0.15% Triton Dilution : 10µg/ml
| Manufacturer: | GeneTex |
| Preservative: | 0.02% Sodium azide|0.02% Sodium azide |
| Molecular Weight: | 142 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | dynactin subunit 1 , DAP-150 , DP-150 , P135 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Goat |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Peptide with sequence C-QEQLHQLHSRLIS, from the C Terminus of the protein sequence according to NP_004073.2; NP_075408.1; NP_001128512.1; NP_001128513.1; NP_001177765.1; NP_001177766.1. |
| Application: | FC, ICC, IF, IHC-P, WB |
Description
Description: This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]
Additional Text
Gene Name
DCTN1
Concentration
0.5 mg/ml
Uniprot ID
Q14203
Gene ID
1639
Molecular Weight
142
Purification
Ammonium Sulfate Precipitated; Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1-2µg/ml. ICC/IF: 10µg/ml. IHC-P: 2-4µg/ml. FCM: 10µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX11806 Datasheet
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