AKT (phospho Ser473) antibody [104A282]

Catalogue Number: GTX11901-GTX

Manufacturer:GeneTex
Preservative:0.05% Sodium azide
Physical state:Liquid
Type:Monoclonal Primary Antibody - Unconjugated
Alias:AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA
Shipping Condition:Blue Ice
Unit(s): 50 ug
Host name: Mouse
Clone: 104A282
Isotype: IgG1
Immunogen: This monoclonal antibody was raised against a synthetic peptide containing phosphorylated serines at amino acid residues 473 of human Akt1.
Application: ICC, IF, IHC-P, WB

Description

Description: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Additional Text

Gene Name

AKT1

Concentration

1 mg/ml

Gene ID

207

Uniprot ID

P31749

Purification

Protein G purified

Modification

Phosphorylated, Ser473

Molecular Weight

56

Antibody Clonality

Monoclonal

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption

Application Notes

IHC-P: 1:200 - 1:250. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.