KDM6A antibody [N2C1], Internal
Catalogue Number: GTX121246-GTX
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Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using KDM6A(GTX121246) antibody at 1:500 dilution.
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KDM6A antibody [N2C1], Internal immunoprecipitates KDM6A protein in IP experiments. IP samples: SK-N-SH whole cell extract A. 30 µg SK-N-SH whole cell extract B. Control with 4 µg of preimmune Rabbit IgG C. Immunoprecipitation of KDM6A protein by 4 µg KDM6A antibody [N2C1], Internal (GTX121246) 5 % SDS-PAGE The immunoprecipitated KDM6A protein was detected by KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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ChIP was performed with HeLa chromatin extract and 5 µg of either normal rabbit IgG or anti-KDM6A antibody. The precipitated DNA was detected by PCR with primer set targeting to RBL1 promoter.
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Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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KDM6A antibody [N2C1], Internal detects KDM6A protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. KDM6A stained by KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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KDM6A antibody [N2C1], Internal detects KDM6A protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. KDM6A stained by KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Non-transfected (-) and transfected (+) U87-MG whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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The data was published in the journal Nat Commun in 2017. PMID: 28387316
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Immunohistochemical analysis of paraffin-embedded H441 xenograft, using KDM6A(GTX121246) antibody at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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KDM6A antibody [N2C1], Internal detects KDM6A protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. KDM6A antibody [N2C1], Internal (GTX121246) dilution: 1:1000. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with KDM6A antibody [N2C1], Internal (GTX121246) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 154 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | lysine demethylase 6A , KABUK2 , UTX , bA386N14.2 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human KDM6A. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB |
Description
Description: This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. [provided by RefSeq]
Additional Text
Uniprot ID
O15550
Gene Name
Kdm6a
Concentration
0.78 mg/ml
Purification
Affinity Purified
Gene ID
7403
Molecular Weight
154
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX121246 Datasheet
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