IKB alpha antibody [6A920]
Catalogue Number: GTX12134-GTX
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IHC-P analysis of human breast tissue using GTX12134 IKB alpha antibody [6A920]. Dilution : 5 µg/ml Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0
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FACS (Intracellular staining) analysis of THP-1 cells using GTX12134 IKB alpha antibody [6A920]. Red : Primary antibody Green : isotype control Shaded histogram : cell only Antibody amount : 0.25µg/10^6 cells
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WB analysis of (A) Daudi and (B) NIH 3T3 cell lysate using GTX12134 IKB alpha antibody [6A920]. Dilution : 2 µg/ml
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WB analysis of 100 µg/ml ALLN* (N-Acetyl-Leu-Leu-Norleucinal) treated Jurkat cell followed by incubation with (lanes 2 & 4) or without 1 nM TNF-a (1 & 3) using GTX12134 IKB alpha antibody [6A920] and IKB alpha phospho-specific antibody. *ALLN (N-Acetyl-Leu-Leu-Norleucinal) : Calpain inhibitor and also proteasome inhibitor that prevents IkBa dephosphorylation
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WB analysis of Jurkat, LNCaP, PC-3, HeLa, NIH3T3 cell lysates using GTX12134 IKB alpha antibody [6A920]. Dilution : 0.5 µg/ml
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WB analysis of NIH-3T3 cell lysate using GTX12134 IKB alpha antibody [6A920].
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 36 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | NFKB inhibitor alpha , EDAID2 , IKBA , MAD-3 , NFKBI |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | 6A920 |
| Isotype: | IgG1 |
| Immunogen: | A recombinant protein corresponding to amino acid residues 32-291 of human IkBa was used as immunogen. |
| Application: | FC, ICC, IF, IHC-P, IP, WB, MS |
Description
Description: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
Additional Text
Gene ID
4792
Gene Name
NFKBIA
Uniprot ID
P25963
Purification
Protein G purified
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
36
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1 - 2 µg/ml. ICC/IF: 1:20 - 1:1000. IHC-P: 1:100. FCM: 0.25 - 1 µg / 10⁶ cells. IP: 1 µg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX12134 Datasheet
SUPPORT
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PRODUCT
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DELIVERY
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