IKK beta antibody [10AG2]
Catalogue Number: GTX12139-GTX
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GTX12139 Image
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GTX12139 Image
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IHC-P analysis of human gallbladder tissue using GTX12139 IKK beta antibody [10AG2]. Dilution : 5 µg/ml Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0
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FACS (Intracellular staining) analysis of HEK293 cells using GTX12139 IKK beta antibody [10AG2]. Red : primary antibody Green : isotype control Antibody amount : 0.5µg
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FACS (Intracellular staining) analysis of HeLa cells using GTX12139 IKK beta antibody [10AG2]. Blue : Primary antibody Orange : isotype control Fixation : 4% PFA Permibilization : 0.1% saponin Antibody amount : 1µg/100 µl buffer
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FACS (Intracellular staining) analysis of Jurkat cells using GTX12139 IKK beta antibody [10AG2]. Blue : Primary antibody Orange : isotype control Fixation : 4% PFA
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FACS analysis of HEK293 cells using GTX12139 IKK beta antibody [10AG2]. Red : primary antibody Green : isotype control Antibody amount : 0.1µg
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WB analysis of (A) Daudi, B) HeLa, and C) NIH3T3 cell lysate using GTX12139 IKK beta antibody [10AG2]. Dilution : 2 µg/ml
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WB analysis of HeLa cell lysate using GTX12139 IKK beta antibody [10AG2].
| Manufacturer: | GeneTex |
| Preservative: | 0.05% Sodium azide|0.05% Sodium azide |
| Molecular Weight: | 87 |
| Physical state: | Liquid |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | inhibitor of nuclear factor kappa B kinase subunit beta , IKK-beta , IKK2 , IKKB , IMD15 , IMD15A , IMD15B , NFKBIKB |
| Shipping Condition: | Blue Ice |
| Unit(s): | 50 ug |
| Host name: | Mouse |
| Clone: | 10AG2 |
| Isotype: | IgG1 |
| Immunogen: | A full-length human IKKb recombinant protein was used to immunize mice (NP_001547). |
| Application: | FC, ICC, IF, IHC-P, IP, WB, MS |
Description
Description: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
Additional Text
Gene Name
IKBKB
Gene ID
3551
Uniprot ID
O14920
Purification
Protein G purified
Molecular Weight
87
Antibody Clonality
Monoclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 2 - 4 µg/ml. IHC-P: 1:10 - 1:500. FCM: 0.25 - 0.5 µg / 10⁶ cells. IP: 1:10 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX12139 Datasheet
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DELIVERY
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