TET3 antibody [C3], C-term
Catalogue Number: GTX121453-GTX
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TET3 antibody [C3], C-term detects TET3 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET3 protein stained by TET3 antibody [C3], C-term (GTX121453) diluted at 1:400. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
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TET3 antibody immunoprecipitates TET3 protein in IP experiments. IP Sample: cell lysate/extract of TET3 gene transfected 293T cells A. Cell lysate/extract of transfected 293T cell B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of TET3 by 2 µg of TET3 antibody (GTX121453) 5% SDS-PAGE The immunoprecipitated TET3 protein was detected by TET3 antibody (GTX121453) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term (GTX121453) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET3 antibody [C3], C-term (GTX121453) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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The data was published in the journal Int J Endocrinol in 2020.PMID: 32089682
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The data was published in the journal Oncogene in 2019. PMID: 31089260
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 179 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | tet methylcytosine dioxygenase 3 , hCG_40738 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the C-terminus region of human TET3. The exact sequence is proprietary. |
| Application: | ICC, IF, IP, ChIP, WB, IHC-WM |
Description
Description: Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM, Nov 2010]
Additional Text
Gene Name
TET3
Uniprot ID
O43151
Purification
Affinity Purified
Gene ID
200424
Molecular Weight
179
Concentration
0.14 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Short Description
TET3 antibody recognizes TET3 protein (predicted molecular weight of 194 kDa), a dioxygenase involved in active DNA demethylation that converts 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) and subsequently to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). Like TET1 and TET2, TET3 appears to impact epigenetic regulation of gene expression. It is also involved in early embryogenesis, neuronal function, and other processes.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Application Notes
WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IP: 1:500-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
GTX121453 Datasheet
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