Mre11 antibody
Catalogue Number: GTX12159-GTX
GTX12159 Image
GTX12159 Image
Chromatin Immunoprecipitation (ChIP) using GeneTex Affinity Purified Mre11 (S. cerevisiae) antibody (GTX12159). A yeast strain containing the HO endonuclease gene controlled by a galactose-inducible promoter (uninduced 0 m lanes) was shifted into galactose containing medium (induced 60 m lanes). After 1 hour of induction cells were cross-linked with formaldehyde followed by preparation of sheared chromatin. Chromatin was immunoprecipitated with the antibody at the stated dilutions. Immunocomplexes were captured using polyacrylamide bead linked secondary antibodies. The resultant immunoprecipitate was probed by multiplex PCR, using primers 20 bp from the MAT locus double strand break (lower arrow) and 67 kb from the break (upper band, control locus). PCR products were displayed on a polyacrylamide gel, stained with SyBR GreenR (Invitrogen), and detected using a Fuji scanning fluorometer.
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Sodium azide |
| Molecular Weight: | 81 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | MRE11 homolog, double strand break repair nuclease , ATLD , HNGS1 , MRE11A , MRE11B |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to an internal region near amino acids 575-600 of Saccharomyces cerevisiae (baker's yeast) Mre11 protein. |
| Application: | ELISA, ChIP, WB |
Description
Description: Mre11 is an 80 kDa protein that acts as a Mn2+ dependent double stranded DNA 3' to 5' exonuclease and a single stranded DNA endonuclease. It is part of a larger complex involved in the nucleolytic end-processing of double strand breaks and telomere maintenance.
Additional Text
Gene ID
4361
Uniprot ID
P49959
Concentration
1.17 mg/mL
Gene Name
MRE11
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
81
Application Notes
WB: 1:500-1:2000. ELISA: 1:10000-1:50000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX12159 Datasheet
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