NFYB antibody
Catalogue Number: GTX121922-GTX
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Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX121922 Image
Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or anti-NFYB antibody. The precipitated DNA was detected by PCR with primer set targeting to aurora kinase A.
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Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using NFYB antibody (GTX121922) at 1:300 dilution.
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Immunohistochemical analysis (whole mount) of zebrafish embryo, using NFYB antibody (GTX121922) at 1:200 dilution.
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NFYB antibody immunoprecipitates NFYB protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 40 µg whole cell lysate/extract of NFYB protein expressing 293T cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of NFYB protein by 2.5 µg of NFYB antibody (GTX121922) 10% SDS-PAGE The immunoprecipitated NFYB protein was detected by NFYB antibody (GTX121922) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
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HeLa whole cell and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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NFYB antibody detects NFYB protein at nucleus in mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. NFYB antibody (GTX121922) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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NFYB antibody detects NFYB protein at nucleus in rat stomach by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. NFYB antibody (GTX121922) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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NFYB antibody detects NFYB protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFYB protein stained by NFYB antibody (GTX121922) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar = 10 µm.
GTX121922 Image
Various tissue extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (GTX121922) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 23 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | nuclear transcription factor Y subunit beta , CBF-A , CBF-B , HAP3 , NF-YB |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB, IHC-WM |
Description
Description: The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq]
Additional Text
Gene ID
4801
Gene Name
NFYB
Uniprot ID
P25208
Concentration
0.42 mg/ml
Purification
Affinity Purified
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Molecular Weight
23
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX121922 Datasheet
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