SMYD3 antibody
Catalogue Number: GTX121956-GTX
GTX121956 Image
GTX121956 Image
GTX121956 Image
GTX121956 Image
GTX121956 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody (GTX121956) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX121956 Image
Cross-linked ChIP was performed with HepG2 chromatin extract and 5 µg of either control rabbit IgG or anti-SMYD3 antibody. The precipitated DNA was detected by PCR with primer set targeting to 5' flanking region of Nkx2.8.
GTX121956 Image
SMYD3 antibody immunoprecipitates SMYD3 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : Control with 2.5 µg of pre-immune rabbit IgG B : Immunoprecipitation of SMYD3 by 2.5 µg of SMYD3 antibody (GTX121956) 10% SDS-PAGE The immunoprecipitated SMYD3 protein was detected by SMYD3 antibody (GTX121956) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
GTX121956 Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SMYD3(GTX121956) antibody at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 49 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | SET and MYND domain containing 3 , KMT3E , ZMYND1 , ZNFN3A1 , bA74P14.1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human SMYD3. The exact sequence is proprietary. |
| Application: | IHC-P, IP, ChIP, WB |
Description
Description: SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.[supplied by OMIM]
Additional Text
Gene Name
SMYD3
Uniprot ID
Q9H7B4
Purification
Affinity Purified
Gene ID
64754
Molecular Weight
49
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX121956 Datasheet
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