ALDH1A1 antibody
Catalogue Number: GTX123973-GTX
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PG-4 whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody (GTX123973) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody (GTX123973) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunofluorescence analysis of methanol-fixed A549, using ALDH1A1(GTX123973) antibody at 1:500 dilution.
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ALDH1A1 antibody detects Aldh1a2 protein on whole-mount zebrafish embryos by immunohistochemical analysis.? Sample: Paraformaldehyde-fixed zebrafish embryos. ALDH1A1 antibody (GTX123973) dilution: 1:200.
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ALDH1A1 antibody detects ALDH1A1 protein by western blot analysis. A. 50 µg mouse liver lysate/extract 10% SDS-PAGE ALDH1A1 antibody (GTX123973) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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ALDH1A1 antibody detects ALDH1A1 protein by western blot analysis. A. 50 µg rat liver lysate/extract 10% SDS-PAGE ALDH1A1 antibody (GTX123973) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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The data was published in the journal Cell Death Dis in 2015. PMID: 26136074
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The data was published in the 2021 in Cancer Drug Resist. PMID: 35582384
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The data was published in the 2020 in Front Pharmacol. PMID: 33381038
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The data was published in the journal Mol Oncol in 2020.PMID: 32037723
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Immunohistochemical analysis of paraffin-embedded human gastric cancer, using ALDH1A1(GTX123973) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 55 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | aldehyde dehydrogenase 1 family member A1 , ALDC , ALDH-E1 , ALDH1 , ALDH11 , HEL-9 , HEL-S-53e , HEL12 , PUMB1 , RALDH1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human ALDH1A1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC-WM |
Description
Description: This protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a cytosolic isoform, which has a high affinity for aldehydes. [provided by RefSeq]
Additional Text
Gene Name
ALDH1A1
Gene ID
216
Uniprot ID
P00352
Purification
Affinity Purified
Molecular Weight
55
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX123973 Datasheet
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