TET2 antibody [N2], N-term
Catalogue Number: GTX124204-GTX
GTX124204 Image
GTX124204 Image
GTX124204 Image
GTX124204 Image
GTX124204 Image
GTX124204 Image
GTX124204 Image
TET2 antibody [N2], N-term detects TET2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET2 protein stained by TET2 antibody [N2], N-term (GTX124204) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 µm.
GTX124204 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2], N-term (GTX124204) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunoprecipitation of TET2 protein from 293T whole cell extracts using 5 µg of TET2 antibody [N2], N-term (GTX124204). Western blot analysis was performed using TET2 antibody [N2], N-term (GTX124204) diluted at 1:500. EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.
GTX124204 Image
Wild-type (WT) and TET2 knockout (KO) HeLa cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2], N-term (GTX124204) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
GTX124204 Image
TET2 antibody detects TET2 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells 5% SDS-PAGE TET2 antibody (GTX124204) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Cross-linked ChIP was performed with U2OS chromatin extract and 5 µg of either control rabbit IgG or anti-TET2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 224 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | tet methylcytosine dioxygenase 2 , KIAA1546 , MDS |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human TET2. The exact sequence is proprietary. |
| Application: | ICC, IF, IP, ChIP, WB |
Additional Text
Gene Name
TET2
Uniprot ID
Q6N021
Purification
Affinity Purified
Gene ID
54790
Molecular Weight
224
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX124204 Datasheet
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