TET2 antibody [N2-2], N-term
Catalogue Number: GTX124205-GTX
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Wild-type (WT) and TET2 knockout (KO) HeLa cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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TET2 antibody detects TET2 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells 5% SDS-PAGE TET2 antibody (GTX124205) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunoprecipitation of TET2 protein from 293T whole cell extracts using 5 µg of TET2 antibody [N2-2], N-term (GTX124205). Western blot analysis was performed using TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.
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TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Cross-linked ChIP was performed with U2OS chromatin extract and 5 µg of either control rabbit IgG or anti-TET2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.
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TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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TET2 antibody [N2-2], N-term detects TET2 protein on human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded cervical carcinoma. TET2 antibody [N2-2], N-term (GTX124205) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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HeLa whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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TET2 antibody [N2-2], N-term detects TET2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET2 stained by TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2-2], N-term (GTX124205) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 224 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | tet methylcytosine dioxygenase 2 , KIAA1546 , MDS |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human TET2. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB |
Description
Description: The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. The encoded protein is involved in myelopoiesis, and defects in this gene have been associated with several myeloproliferative disorders. Two variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]
Additional Text
Gene Name
TET2
Uniprot ID
Q6N021
Concentration
1.78 mg/mL
Purification
Affinity Purified
Gene ID
54790
Molecular Weight
224
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX124205 Datasheet
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