TET1 antibody [N3C1]
Catalogue Number: GTX124207-GTX
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Non-transfected (-) and transfected (+) 293T whole cell extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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TET1 antibody [N3C1] detects TET1 protein at nucleus by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:1000. Blue: Hoechst 33342 staining.
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TET1 antibody [N3C1] detects TET1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).
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TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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The data was published in the journal Sci Rep in 2016. PMID: 27708396
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The data was published in the journal Clin Epigenetics in 2018. PMID: 30075814
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The data was published in the journal Clin Epigenetics in 2018. PMID: 30075814
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The data was published in the journal Int J Endocrinol in 2020.PMID: 32089682
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The data was published in the journal Int J Endocrinol in 2020.PMID: 32089682
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The data was published in the journal BMC Cancer in 2018.PMID: 30045709
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The data was published in the journal Clin Epigenetics in 2016.PMID: 26973719
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The data was published in the journal Clin Epigenetics in 2016.PMID: 26973719
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TET1 antibody [N3C1] detects TET1 protein at nucleus on Human normal prostate tissue by immunohistochemical analysis. Sample: Paraffin-embedded Human normal prostate tissue. TET1 antibody [N3C1] (GTX124207) dilution: 1:1000. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Immunohistochemical analysis of paraffin-embedded Hela xenograft, using TET1 (GTX124207) antibody at 1:1000 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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TET1 antibody [N3C1] detects TET1 protein at nucleus in human A549 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human A549 xenograft . TET1 antibody [N3C1] (GTX124207) diluted at 1:250. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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HeLa whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX124207 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 235 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | tet methylcytosine dioxygenase 1 , CXXC6 , LCX , bA119F7.1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB, IHC, IHC-WM |
Description
Description: Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.
Additional Text
Gene Name
Tet1
Concentration
1.01 mg/ml
Uniprot ID
Q8NFU7
Purification
Affinity Purified
Gene ID
80312
Molecular Weight
235
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
TET1 antibody recognizes TET1 protein, which is a methylcytosine dioxygenase that catalyzes the sequential steps of DNA demethylation. Expression of TET1 protein is spatiotemporally regulated during embryonic development. Abnormal TET1 activation is associated with cancer growth and metastasis.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX124207 Datasheet
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