beta Actin antibody
Catalogue Number: GTX124214-GTX
GTX124214 Image
GTX124214 Image
GTX124214 Image
GTX124214 Image
GTX124214 Image
GTX124214 Image
GTX124214 Image
GTX124214 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (10 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX124214) diluted at 1:15000.
GTX124214 Image
Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with beta Actin antibody (GTX124214) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX124214 Image
Immunofluorescence analysis of methanol-fixed HeLa, using beta actin(GTX124214) antibody at 1:500 dilution.
GTX124214 Image
Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE GTX124214 diluted at 1:10000
GTX124214 Image
Sample (30 ug of whole cell lysate) A: Jurakt B: Raji C: K562 10% SDS PAGE GTX124214 diluted at 1:10000
GTX124214 Image
beta actin antibody detects beta actin protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. beta actin antibody (GTX124214) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX124214 Image
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using beta actin(GTX124214) antibody at 1:500 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Molecular Weight: | 42 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | actin beta , BRWS1 , PS1TP5BP1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the center region of human beta Actin. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB |
Description
Description: This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq]
Additional Text
Gene Name
ACTB
Molecular Weight
42
Gene ID
60
Purification
Affinity Purified
Uniprot ID
P60709
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX124214 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations