TET1 antibody [N1], N-term
Catalogue Number: GTX125888-GTX
GTX125888 Image
GTX125888 Image
GTX125888 Image
GTX125888 Image
GTX125888 Image
GTX125888 Image
GTX125888 Image
Western blot analysis of 293T cell are mock transfected (lane1) or with tagged-mTet1 (lane 2) for 24hrs, using either GTX125888 (diluted at 1:2000) (A) or anti-tag (B) antibody. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX125888 Image
TET1 antibody detects TET1 protein by western blot analysis. Non-transfected (-) and mouse TET1-transfected (+, including DDDDK-tag) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody (GTX125888) at a dilution of 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX125888 Image
TET1 antibody [N1], N-term detects TET1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. TET1 stained by TET1 antibody [N1], N-term (GTX125888) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX125888 Image
The data was published in the journal Stem Cell Reports in 2018.PMID: 29576538
GTX125888 Image
The data was published in the 2022 in Nucleic Acids Res. PMID: 35150568
GTX125888 Image
The data was published in the journal Oncotarget in 2016. PMID: 27449105
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 219 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | tet methylcytosine dioxygenase 1 , 2510010B09Rik , AA517754 , BB001228 , Cxxc6 , D10Ertd17e , LCX , mKIAA1676 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of mouse TET1. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB, IHC-WM |
Description
Description: Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.
Additional Text
Uniprot ID
Q3URK3
Gene Name
Tet1
Purification
Affinity Purified
Molecular Weight
219
Gene ID
52463
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX125888 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations