N-Cadherin antibody
Catalogue Number: GTX127345-GTX
GTX127345 Image
GTX127345 Image
GTX127345 Image
GTX127345 Image
GTX127345 Image
GTX127345 Image
GTX127345 Image
GTX127345 Image
GTX127345 Image
GTX127345 Image
GTX127345 Image
GTX127345 Image
GTX127345 Image
GTX127345 Image
N-Cadherin antibody detects N-Cadherin protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). N-Cadherin antibody (GTX127345) diluted at 1:500.
GTX127345 Image
N-Cadherin antibody detects N-Cadherin protein at cell membrane by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: N-Cadherin protein stained by N-Cadherin antibody (GTX127345) diluted at 1:500. Blue: Hoechst 33342 staining.
GTX127345 Image
N-Cadherin antibody detects N-Cadherin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: N-Cadherin protein stained by N-Cadherin antibody (GTX127345) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
GTX127345 Image
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX127345 Image
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX127345 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
GTX127345 Image
Wild-type (WT) and N-Cadherin knockout (KO) 293T cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX127345 Image
N-Cadherin antibody detects N-Cadherin protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. N-Cadherin stained by N-Cadherin antibody (GTX127345) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX127345 Image
Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody (GTX127345) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX127345 Image
The data was published in the 2022 in Front Pharmacol. PMID: 35517780
GTX127345 Image
The data was published in the 2022 in Front Pharmacol. PMID: 35517780
GTX127345 Image
The data was published in the 2022 in Front Pharmacol. PMID: 35517780
GTX127345 Image
The data was published in the 2022 in Front Pharmacol. PMID: 35517780
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 100 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | cadherin 2 , CD325 , CDHN , CDw325 , NCAD |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide encompassing a sequence within the Intracellular domain of human N-Cadherin. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, WB, IHC-Fr |
Description
Description: This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]
Additional Text
Gene Name
CDH2
Gene ID
1000
Uniprot ID
P19022
Concentration
0.15 mg/ml
Purification
Affinity Purified
Molecular Weight
100
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Short Description
N-Cadherin antibody recognizes N-cadherin protein, a calcium-dependent cell adhesion protein (predicted molecular weight of 100 kDa) encoded by the CDH2 gene. N-cadherin is involved in multiple processes, including neuron development and epithelial-mesenchymal transition (EMT). Due to the prominent role of EMT in cancer progression and metastasis, antibodies targeting either N-cadherin or E-cadherin are frequently used together to study the EMT process: the upregulation of N-cadherin followed by the downregulation of E-cadherin.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX127345 Datasheet
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