Histone H3S10ph (phospho Ser10) antibody
Catalogue Number: GTX128116-GTX
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Histone H3 (phospho Ser10) antibody detects Histone H3 (phospho Ser10) protein at chromosome by confocal immunofluorescent analysis. Sample: Mitotic HeLa cells were fixed in 2% paraformaldehyde for 30 min. Green: Histone H3 (phospho Ser10) protein stained by Histone H3 (phospho Ser10) antibody (GTX128116) diluted at 1:500. Red: alpha Tubulin antibody (GTX11304) diluted at 1:2500. Blue: Hoechst 33342 staining. [Images captured by Olympus FV1000 Confocal Laser Scanning Microscope]
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Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein on zebrafish by whole mount immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo. Histone H3S10ph (phospho Ser10) antibody (GTX128116) dilution: 1:100.
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Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein on zebrafish by whole mount immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo. Histone H3S10ph (phospho Ser10) (GTX128116) antibody dilution: 1:100.
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Cross-linked ChIP was performed with HeLa chromatin extract treated with Nocodazole (100 ng/ml for 24 h) and 5 µg of either control rabbit IgG or anti-Histone H3S10ph (phospho Ser10) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH promoter.
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HIST1H3A antibody histone cluster 1, H3a protein by immunofluorescent analysis. Sample: RFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: histone cluster 1, H3a protein stained by HIST1H3A antibody (GTX128116) diluted at 1:500. Red: Tubulin, alpha 1b protein stained by TUBA1B antibody (GTX11304) diluted at 1:500 . Blue: Hoechst 33342 staining.
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Histone H3S10ph (phospho Ser10) antibody immunoprecipitates HIST1H3B protein in IP experiments. IP samples: 293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr A. 30 µg 293T whole cell extract treat with 100ng/ml Nocodazole for 24 hr B. Control with 4 µg of preimmune Rabbit IgG C. Immunoprecipitation of HIST1H3B protein by 4 µg Histone H3S10ph (phospho Ser10) antibody (GTX128116) 15 % SDS-PAGE The immunoprecipitated HIST1H3B protein was detected by Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:1000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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Histone H3 (phospho Ser10) antibody detects HIST1H3A protein by western blot analysis. A. 30 µg 4T1 whole lysate/extract (untreated) B. 30 µg 4T1 whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr) 15% SDS-PAGE Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Histone H3 (phospho Ser10) antibody detects Histone H3 (phospho Ser10) protein by western blot analysis. A. 30 µg Rat2 whole cell lysate/extract (untreated) B. 30 µg Rat2 whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr) 15% SDS-PAGE Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Histone H3S10ph (phospho Ser10) antibody detects Histone H3S10ph (phospho Ser10) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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The data was published in the journal Sci Rep in 2016. PMID: 27819330
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Histone H3 (phospho Ser10) antibody detects HIST1H3A protein at nucleus on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. Histone H3 (phospho Ser10) antibody (GTX128116) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Untreated (-) and treated (+) HeLa whole cell extract (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3S10ph (phospho Ser10) antibody (GTX128116) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide surrounding phospho Ser10 of human Histone H3. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB, IHC-WM |
Additional Text
Concentration
0.19 mg/ml
Purification
Affinity Purified
Modification
Phosphorylated, Ser10
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Wm: 1:100-1:500. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX128116 Datasheet
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