Histone H3K4me3 (Tri-methyl Lys4) antibody
Catalogue Number: GTX128954-GTX
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ChIP was performed with HeLa chromatin extract and 5 µg of either normal rabbit IgG or anti-Histone H3K4me3 (trimethyl Lys4) antibody. The precipitated DNA was detected by PCR with primer set targeting to GADPH gene.
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Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein on zebrafish by whole mount immunohistochemical analysis. Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo. Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:100.
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Whole zebrafish extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me3 antibody (GTX128954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Dotblot analysis of anti-Histone H3 (tri-Methyl Lys4) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (tri-Methyl Lys4) antibody (GTX128954) 1:5000 dilution. A: Peptide samples of H3K4 un B: Peptide samples of H3K4me1 C: Peptide samples of H3K4me2 D: Peptide samples of H3K4me3 E: Peptide samples of H3K4ac
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Histone H3 (tri-Methyl Lys4) antibody detects Histone H3 (tri-Methyl Lys4) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3 (tri-Methyl Lys4) protein stained by Histone H3 (tri-Methyl Lys4) antibody (GTX128954) diluted at 1:500. Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Blue: Hoechst 33342 staining.
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Histone H3K4me3 (Tri-methyl Lys4) antibody detects Histone H3K4me3 (Tri-methyl Lys4) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. Histone H3K4me3 (Tri-methyl Lys4) stained by Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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Histone H3K4me3 (Tri-methyl Lys4) antibody detects Histone H3K4me3 (Tri-methyl Lys4) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. Histone H3K4me3 (Tri-methyl Lys4) stained by Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX128954 Image
Histone H3K4me3 (Tri-methyl Lys4) antibody detects Histone H3K4me3 (Tri-methyl Lys4) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K4me3 (Tri-methyl Lys4) stained by Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
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Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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Histone H3K4me3 (trimethyl Lys4) antibody detects Histone H3K4me3 (trimethyl Lys4) protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Histone H3K4me3 (trimethyl Lys4) antibody (GTX128954) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
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HeLa whole cell and nuclear extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K4me3 (Tri-methyl Lys4) antibody (GTX128954) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide surrounding tri-methyl Lys4 of human Histone H3. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, ChIP, EM, WB, IHC-Fr, DB, IHC-WM |
Additional Text
Concentration
0.12 mg/ml
Purification
Affinity Purified
Modification
Trimethylated, Lys4
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Wm: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX128954 Datasheet
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