Histone H2AK5ac (acetyl Lys5) antibody
Catalogue Number: GTX128961-GTX
GTX128961 Image
GTX128961 Image
GTX128961 Image
GTX128961 Image
GTX128961 Image
Histone H2AK5ac (acetyl Lys5) antibody detects Histone H2AK5ac (acetyl Lys5) protein at nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 0.4 µM TSA for 18 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H2AK5ac (acetyl Lys5) protein stained by Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
GTX128961 Image
Dotblot analysis of anti-Histone H2AK5ac (acetyl Lys5) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) 1:5000 dilution. A: Peptide samples of H2A.2K5 un B: Peptide samples of H2A.2K5ac C: Peptide samples of H2BK20 un D: Peptide samples of H2BK20ac E: Peptide samples of H2BK5 un F: Peptide samples of H2BK5ac
GTX128961 Image
Histone H2AK5ac (acetyl Lys5) antibody immunoprecipitates Histone H2AK5ac (acetyl Lys5) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 µg whole cell lysate/extract of Histone H2AK5ac (acetyl Lys5) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of Histone H2AK5ac (acetyl Lys5) protein by 2.5 µg of Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) 12% SDS-PAGE The immunoprecipitated Histone H2AK5ac (acetyl Lys5) protein was detected by Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) diluted at 1 : 10000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
GTX128961 Image
Histone H2AK5ac (acetyl Lys5) antibody detects Histone H2AK5ac (acetyl Lys5) protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr) B. 30 µg HeLa whole cell lysate/extract (0.4 µM Trichostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) dilution: 1:10000
| Manufacturer: | GeneTex |
| Preservative: | 0.01% Thimerosal|0.01% Thimerosal |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide surrounding acetyl Lys5 of human Histone H2A. The exact sequence is proprietary. |
| Application: | ICC, IF, IP, WB, DB |
Additional Text
Purification
Affinity Purified
Gene ID
8337
Modification
Acetylated, Lys5
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Gene Name
H2AC18
Concentration
1 mg/ml
Application Notes
WB: 1:1000-1:20000. ICC/IF: 1:100-1:1000. IP: 1:100-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX128961 Datasheet
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