DNMT3A antibody
Catalogue Number: GTX129125-GTX
GTX129125 Image
GTX129125 Image
GTX129125 Image
GTX129125 Image
GTX129125 Image
GTX129125 Image
GTX129125 Image
GTX129125 Image
GTX129125 Image
GTX129125 Image
GTX129125 Image
GTX129125 Image
GTX129125 Image
GTX129125 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX129125 Image
Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX129125 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX129125 Image
Immunoprecipitation of DNMT3A protein from 293T whole cell extracts using 5 µg of DNMT3A antibody (GTX129125) or DNMT3A antibody (GTX129126). Western blot analysis was performed using DNMT3A antibody (GTX129125) diluted at 1:2000. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX129125 Image
DNMT3A antibody immunoprecipitates DNMT3A protein in IP experiments. IP samples: 293T nuclear extract A. 50 µg 293T nuclear extract B. Control with 4 µg of preimmune Rabbit IgG C. Immunoprecipitation of DNMT3A protein by 4 µg DNMT3A antibody (GTX129125) 5 % SDS-PAGE The immunoprecipitated DNMT3A protein was detected by DNMT3A antibody (GTX129125) diluted at 1:2000. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
GTX129125 Image
Wild-type (WT) and DNMT3A knockout (KO) 293T cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX129125 Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX129125 Image
DNMT3A antibody detects DNMT3A protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: DNMT3A stained by DNMT3A antibody (GTX129125) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
GTX129125 Image
DNMT3A antibody detects DNMT3A protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. DNMT3A stained by DNMT3A antibody (GTX129125) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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The data was published in the journal PLoS One in 2015. PMID: 25928539
GTX129125 Image
The data was published in the journal Oncotarget in 2018. PMID: 29416619
GTX129125 Image
The data was published in the journal Oncotarget in 2018. PMID: 29416619
GTX129125 Image
DNMT3A antibody detects DNMT3A protein at nucleus on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. DNMT3A antibody (GTX129125) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 102 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | DNA methyltransferase 3 alpha , DNMT3A2 , M.HsaIIIA , TBRS |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human DNMT3A. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, EM, WB |
Description
Description: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
Additional Text
Gene Name
DNMT3A
Gene ID
1788
Concentration
1.28 mg/mL
Purification
Affinity Purified
Molecular Weight
102
Uniprot ID
Q9Y6K1
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX129125 Datasheet
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