SIN3A antibody
Catalogue Number: GTX129156-GTX
GTX129156 Image
GTX129156 Image
GTX129156 Image
GTX129156 Image
GTX129156 Image
GTX129156 Image
GTX129156 Image
GTX129156 Image
GTX129156 Image
GTX129156 Image
Various tissue extracts (50 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SIN3A antibody (GTX129156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX129156 Image
Cross-linked ChIP was performed with MCF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 µg of either control rabbit IgG or anti-SIN3A antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or MyoD.
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SIN3A antibody immunoprecipitates SIN3A protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 µg whole cell lysate/extract of SIN3A protein expressing 293T cells B : Control with 3 µg of pre-immune rabbit IgG C : Immunoprecipitation of SIN3A by 3 µg of SIN3A antibody (GTX129156) 5% SDS-PAGE The immunoprecipitated SIN3A protein was detected by SIN3A antibody (GTX129156) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
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SIN3A antibody detects SIN3A protein by western blot analysis. A 293T whole cell lysate/extract B A431 whole cell lysate/extract C HeLa whole cell lysate/extract D HepG2 whole cell lysate/extract 5% SDS-PAGE SIN3A antibody (GTX129156) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX129156 Image
HeLa whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SIN3A antibody (GTX129156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX129156 Image
SIN3A antibody detects SIN3A protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SIN3A stained by SIN3A antibody (GTX129156) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar= 10 µm.
GTX129156 Image
SIN3A antibody detects SIN3A protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. SIN3A antibody (GTX129156) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX129156 Image
SIN3A antibody detects SIN3A protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. SIN3A antibody (GTX129156) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GTX129156 Image
SIN3A antibody detects SIN3A protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. SIN3A antibody (GTX129156) dilution: 1:500. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 145 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | SIN3 transcription regulator family member A , WITKOS |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human SIN3A. The exact sequence is proprietary. |
| Application: | ICC, IF, IHC-P, IP, ChIP, WB |
Description
Description: The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex. [provided by RefSeq]
Additional Text
Gene Name
SIN3A
Uniprot ID
Q96ST3
Concentration
1.38 mg/mL
Purification
Affinity Purified
Gene ID
25942
Molecular Weight
145
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX129156 Datasheet
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