Ikaros antibody
Catalogue Number: GTX129438-GTX
GTX129438 Image
GTX129438 Image
GTX129438 Image
GTX129438 Image
Cross-linked ChIP was performed with Jurkat chromatin extract and 5 µg of either control rabbit IgG or anti-IKZF1 antibody. The precipitated DNA was detected by PCR with primer set targeting to VPAC-1 receptor promoter.
GTX129438 Image
IKZF1 antibody immunoprecipitates IKZF1 protein in IP experiments. IP samples: Raji whole cell extract A. Control with 4 µg of preimmune Rabbit IgG B. Immunoprecipitation of IKZF1 protein by 4 µg IKZF1 antibody (GTX129438) 7.5 % SDS-PAGE The immunoprecipitated IKZF1 protein was detected by IKZF1 antibody (GTX129438) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
GTX129438 Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ikaros antibody (GTX129438) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 58 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | IKAROS family zinc finger 1 , CVID13 , Hs.54452 , IK1 , IKAROS , LYF1 , LyF-1 , PPP1R92 , PRO0758 , ZNFN1A1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human Ikaros. The exact sequence is proprietary. |
| Application: | IP, ChIP, WB |
Description
Description: Transcription regulator of hematopoietic cell differentiation. Binds gamma-satellite DNA. Binds with higher affinity to gamma satellite A. Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (terminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs).
Additional Text
Gene Name
IKZF1
Uniprot ID
Q13422
Concentration
0.38 mg/ml
Purification
Affinity Purified
Gene ID
10320
Molecular Weight
58
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX129438 Datasheet
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