TRRAP antibody
Catalogue Number: GTX129505-GTX
GTX129505 Image
GTX129505 Image
GTX129505 Image
GTX129505 Image
GTX129505 Image
GTX129505 Image
Cross-linked ChIP was performed with HeLa chromatin extract and 5 µg of either control rabbit IgG or anti-TRRAP antibody. The precipitated DNA was detected by PCR with primer set targeting to nucleolin promoter.
GTX129505 Image
TRRAP antibody detects TRRAP protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRRAP protein stained by TRRAP antibody (GTX129505) diluted at 1:1000. Blue: Hoechst 33342 staining.
GTX129505 Image
TRRAP antibody immunoprecipitates TRRAP protein in IP experiments. IP samples: 293T whole cell extract A. Control with 4 µg of preimmune Rabbit IgG B. Immunoprecipitation of TRRAP protein by 4 µg TRRAP antibody (GTX129505) C : Immunoprecipitation of TRRAP by 4 µg of TRRAP antibody (GTX129507) 5 % SDS-PAGE The immunoprecipitated TRRAP protein was detected by TRRAP antibody (GTX129505) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
GTX129505 Image
TRRAP antibody detects TRRAP protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 5 % SDS-PAGE TRRAP antibody (GTX129505) dilution: 1:500
GTX129505 Image
TRRAP antibody detects TRRAP protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg NCI-H929 whole cell lysate/extract 5 % SDS-PAGE TRRAP antibody (GTX129505) dilution: 1:500
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 438 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | transformation/transcription domain associated protein , PAF350/400 , PAF400 , STAF40 , TR-AP , Tra1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Recombinant protein encompassing a sequence within the N-terminus region of human TRRAP. The exact sequence is proprietary. |
| Application: | ICC, IF, IP, ChIP, WB |
Description
Description: Adapter protein, which is found in various multiprotein chromatin complexes with histone acetyltransferase activity (HAT), which gives a specific tag for epigenetic transcription activation. Component of the NuA4 histone acetyltransferase complex which is responsible for acetylation of nucleosomal histones H4 and H2A. Plays a central role in MYC transcription activation, and also participates in cell transformation by MYC. Required for p53/TP53-, E2F1- and E2F4-mediated transcription activation. Also involved in transcription activation mediated by the adenovirus E1A, a viral oncoprotein that deregulates transcription of key genes. Probably acts by linking transcription factors such as E1A, MYC or E2F1 to HAT complexes such as STAGA thereby allowing transcription activation. Probably not required in the steps following histone acetylation in processes of transcription activation. May be required for the mitotic checkpoint and normal cell cycle progression.
Additional Text
Gene Name
TRRAP
Uniprot ID
Q9Y4A5
Purification
Affinity Purified
Gene ID
8295
Molecular Weight
438
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Concentration
1 mg/ml
Application Notes
WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX129505 Datasheet
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