YAP1 antibody
Catalogue Number: GTX130444-GTX
GTX130444 Image
GTX130444 Image
GTX130444 Image
GTX130444 Image
GTX130444 Image
GTX130444 Image
GTX130444 Image
YAP1 antibody detects YAP1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX130444) diluted by 1:1000.
GTX130444 Image
Immunoprecipitation of YAP1 protein from HeLa whole cell extracts using 5 µg of YAP1 antibody (GTX130444). Western blot analysis was performed using YAP1 antibody (GTX130444). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
GTX130444 Image
Various tissue extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX130444) diluted at 1:1000.
GTX130444 Image
Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX130444) diluted at 1:500.
GTX130444 Image
Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with YAP1 antibody (GTX130444) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX130444 Image
YAP1 antibody detects YAP1 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human glioblastoma. YAP1 stained by YAP1 antibody (GTX130444) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
| Manufacturer: | GeneTex |
| Preservative: | 0.025% ProClin 300|0.025% ProClin 300 |
| Molecular Weight: | 54 |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | Yes associated protein 1 , COB1 , YAP , YAP2 , YAP65 , YKI |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human YAP1. The exact sequence is proprietary. |
| Application: | IHC-P, IP, WB |
Description
Description: This gene encodes the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. This protein contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction. [provided by RefSeq]
Additional Text
Gene ID
10413
Gene Name
YAP1
Uniprot ID
P46937
Purification
Affinity Purified
Molecular Weight
54
Concentration
1.48 mg/ml
Antibody Clonality
Polyclonal
Note
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption
Application Notes
WB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Storage Note
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4C. For long-term storage, aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
GTX130444 Datasheet
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